|
KMID : 0545120160260111918
|
|
Journal of Microbiology and Biotechnology
2016 Volume.26 No. 11 p.1918 ~ p.1923
|
|
|
Biological Synthesis of Baicalein Derivatives Using Escherichia coli
|
|
Han Da-Hye
Lee Young-Shim
Ahn Joong-Hoon
|
|
|
Abstract
|
|
|
|
Two baicalein derivatives, baicalin and oroxylin A, were synthesized in this study. These derivatives exhibit diverse biological activities, such as anxiolytic and anticancer activities as well as memory enhancement. In order to synthesize baicalin from aglycon baicalein using Escherichia coli, we utilized a glycosyltransferase that regioselectively transfers glucuronic acid from UDP-glucuronic acid to the 7-hydroxy group of baicalein. To increase baicalin productivity, an araA deletion E. coli mutant, which accumulates UDP-glucuronic acid, was used, and ugd, which converts UDP-glucose to UDP-glucuronic acid, was overexpressed. Using these strategies, approximately 720.3 μM baicalin was synthesized from 1,000 μM baicalein. Oroxylin A was then synthesized from baicalein. Two O-methyltransferases (OMTs), ROMT-15 and POMT-9, were tested to examine the production of oroxylin A from baicalein. E. coli harboring ROMT-15 and E. coli harboring POMT-9 produced reaction products that had different retention times, indicating that they are methylated at different positions; the structure of the reaction product from POMT-9 was consistent with oroxylin A, whereas that from ROMT-15 was 7-O-methyl baicalein. Using E. coli harboring POMT-9, approximately 50.3 mg/l of oroxylin A (177 μM) was synthesized from 54 mg/l baicalein (200 μM).
|
|
|
KEYWORD
|
|
|
baicalein, baicalin, biotransformation, oroxylin A
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|